Takuya SUGAHARA, Ph. D.



Profile

Current Projects

  1. Enhancement of monoclonal antibody production by human-human hybridomas

  2. Development of the large-scale animal cell culture system

  3. Development of the drug delivery system and novel gene vector using lipis vesicles

Selected Publications

  1. Revised stereochemistry of ficifolidione and its biological activities against insects and cells., J. Nat. Prod., In press, 2014.
  2. Methoxychlor enhances degranulation of murine mast cells by regulating FcRI-mediated signal transduction., J. Immunotoxicol., In press, 2014.
    [PubMed 25418051]
  3. Cytotoxic activity of butane type of 1,7-seco-2,7'-cyclolignanes and apoptosis induction by caspase 9 and 3., Bioorg. Med. Chem. Lett., 24, 4231-4235, 2014.
    [PubMed 25124113]
  4. Antiproliferative activity and its underlying mechanisms of methanol extract from leaves of Saururus chinensis (Lour.) Baill. on mouse Colon-26 carcinoma cells., Int. J. Appl. Res. Nat. Prod., 7(3), 17-27, 2014.
  5. Activation of J774.1 murine macrophages by lactate dehydrogenase., Cytotechnology, 66, 937-943, 2014.
    [PubMed 24101442]
  6. The major whey protein -lactoglobulin inhibits IgE-mediated degranulation of RBL-2H3 cells and passive cutaneous anaphylaxis in mice., Int. Dairy J., 39, 89-95, 2014.
    [ScienceDirect]
  7. Studies on anti-allergic activity of Sargassum horneri extract., J. Funct. Foods, 10, 154-160, 2014.
    [ScienceDirect]
  8. Activation of macrophages stimulated by bengkoang fiber extract through Toll-like receptor 4., Food Funct., 5, 1403-1408, 2014.
    [PubMed 24770453]
  9. Tributyltin induces cell cycle arrest at G1 phase in the yeast Saccharomyces cerevisiae., J. Toxicol. Sci., 39, 311-317, 2014.
    [PubMed 24646713]
  10. Nobiletin, a polymethoxy flavonoid, exerts anti-allergic effect by suppressing activation of phosphoinositide 3-kinase., J. Funct. Foods, 6, 606-614, 2014.
    [ScienceDirect]
  11. Jellyfish collagen stimulates production of TNF- and IL-6 by J774.1 cells through activation of NF-kappaB and JNK via TLR4 signaling pathway., Mol. Immunol., 58, 32-37, 2014.
    [PubMed 24291243]
  12. Immunomodulatory activity of bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo., Cytotechnology, 66, 75-85, 2014.
    [PubMed 23361525]
  13. Quantitative structure-activity relationship analysis of antifungal (+)-dihydroguaiaretic acid using 7-phenyl derivatives., J. Agric. Food. Chem., 61, 8546-8555, 2013.
    [PubMed 23924382]
  14. Structure-cytotoxic activity relationship of sesquilignan, Morinol A., Bioorg. Med. Chem. Lett., 23, 4923-4930, 2013.
    [PubMed 23896495]
  15. Phenylhydroquinone induces loss of thymocytes through cell cycle arrest and apoptosis elevation in p53-dependent pathway., J. Toxicol. Sci., 38, 325-335, 2013.
    [PubMed 23665931]
  16. Effect of bengkoang (Pachyrhizus erosus) fiber extract on murine macrophage-like J774.1 cells and mouse peritoneal macrophages., J. Funct. Foods, 5, 204-210, 2013.
    [ScienceDirect]
  17. Inhibitory effect of chlorophyll c2 from brown algae, Sargassum horneri, on degranulation of RBL-2H3 cells., J. Funct. Foods, 5, 204-210, 2013.
    [ScienceDirect]
  18. Inhibitory effect of aqueous spinach extract on degranulation of RBL-2H3 cells., Food Chem., 136, 322-327, 2013.
    [ScienceDirect]
    [PubMed 23122065]
  19. Effect of substituents at phenyl group of 7,7'-dioxo-9,9'-epoxylignane on antifungal activity., Bioorg. Med. Chem. Lett., 22, 6740-6744, 2012.
    [PubMed 23017887]
  20. Concerted actions of the catechol o-methyltransferase and the cytosolic sulfotransferase SULT1A3 in the metabolism of catecholic drugs., Biochem. Pharmacol., 84, 1186-1195, 2012.
    [PubMed 22917559]
  21. Immunomodulatory effects of lactate dehydrogenase in vitro and in vivo., J. Funct. Foods, 4, 972-978, 2012.
    [ScienceDirect]
  22. Immunomodulatory effects of citrus fruit auraptene in vitro and in vivo., J. Funct. Foods, 4, 883-890, 2012.
    [ScienceDirect]
  23. Immunochromatographic assay of cadmium levels in oysters., Talanta, 97, 262-266, 2012.
    [ScienceDirect]
  24. Immunostimulatory effect of beta-cryptoxanthin in vitro and in vivo., J. Funct. Foods, 4, 618-625, 2012.
    [ScienceDirect]
  25. Effect of collagens from jellyfish and bovine Achilles tendon on the activity of J774.1 and mouse peritoneal macrophage Cells., J. Funct. Foods, 4, 504-512, 2012.
    [ScienceDirect]
  26. Immunostimulatory effects of water extract from bulbus arteriosus in tuna in vitro., J. Funct. Foods, 4, 263-270, 2012.
    [ScienceDirect]
  27. Nobiletin suppresses adipocyte differentiation of 3T3-L1 cells by an insulin and IBMX mixture induction., Biochim. Biophys. Acta - Gen. Subjects, 1820, 461-468, 2012.
    [PubMed 22172985]
  28. Evaluation of immunostimulatory effect of the arrowroot (Maranta arundinacea. L) in vitro and in vivo., Cytotechnology, 64, 131-137, 2012.
    [PubMed 22038480]
  29. (-)-Secoisolariciresinol attenuates high-fat diet-induced obesity in C57BL/6 mice., Food Funct., 3, 76-82, 2012.
    [PubMed 22030618]
  30. Immunostimulatory effects of collagen from jellyfish in vivo., Cytotechnology, 63, 481-492, 2011.
    [PubMed 21773823]
  31. Heavy oil fraction induces the dysplastic sperm in male mouse., J. Toxicol. Sci., 36, 487-491, 2011.
    [PubMed 21804313]
  32. Disruption of ion homeostasis by verrucosin and its related compound., Biosci. Biotechnol. Biochem., 75, 1000-1002, 2011.
    [PubMed 21597167]
  33. Immunomodulatory effect of (-)-matairesinol in vivo and ex vivo., Biosci. Biotechnol. Biochem., 75, 859-863, 2011.
    [PubMed 21597179]
  34. Immunostimulation effects of yellowtail heart extracts in vitro and in vivo., Biosci. Biotechnol. Biochem., 75, 638-645, 2011.
    [PubMed 21512243]
  35. Inhibition of myotube formation by paraquat in the myoblast cell line C2C12., J. Toxicol. Sci., 36, 243-246, 2011.
    [PubMed 21467752]
  36. Bfr1p is responsible for tributyltin resistance in Schizosaccharomyces pombe., J. Toxicol. Sci. , 117-120, 2011.
    [PubMed 21297349]
  37. Immunostimulatory in vitro and in vivo effects of a water-soluble extract from kale., Biosci. Biotechnol. Biochem., 75, 40-46, 2011.
    [PubMed 21228486]
  38. Antifungal activity of morinol B derivatives of tetrahydropyran sesquilignan., Biosci. Biotechnol. Biochem., 74, 2071-2076, 2010.
    [PubMed 20944407]
  39. IgE suppressive activity of (-)-matairesinol and structure-activity relationship., Biosci. Biotechnol. Biochem., 74, 1878-1883, 2010.
    [PubMed 20834158]
  40. Improved syntheses of Morinol C and D by employing Mizoroki-Heck reaction and their cytotoxic and antimicrobial activities., Biosci. Biotechnol. Biochem., 74, 1641-1644, 2010.
    [PubMed 20699576]
  41. The effect of secoisolariciresinol on 3T3-L1 adipocytes and the relationship between molecular structure and the activity., Animal Cell Technology: Basic & Applied Aspects, Vol. 16, 345-351, 2010.

  42. The effects of pesticides on immune cells., Animal Cell Technology: Basic & Applied Aspects, Vol. 16, 377-382, 2010.

  43. The differentiation of myoblast cells to myotube by paraquat., Animal Cell Technology: Basic & Applied Aspects, Vol. 16, 215-219, 2010.

  44. Proteose peptone fraction of bovine milk depressed IgE production in vitro and in vivo., Biosci. Biotechnol. Biochem., 74, 1332-1337, 2010.
    [PubMed 20622462]
  45. Zebrafish as a model for the study of the phase II cytosolic sulfotransferases., Curr. Drug Metab., 11, 538-546, 2010.
    [PubMed 20545621]
  46. In vitro and in vivo anti-tumor effects of novel Span 80 vesicles containing immobilized Eucheuma serra agglutinin., Int. J. Pharm., 389, 157-167, 2010.
    [PubMed 20100554]
  47. Oral administration of paraquat perturbs immunoglobulin productivity in mouse., J. Toxicol. Sci., 35, 257-263, 2010.
    [PubMed 20371979]
  48. White sorghum (Sorghum bicolor (L.) Moench) bran extracts suppressed IgE production by U266 cells., Biosci. Biotechnol. Biochem., 73, 2043-2047, 2009.
    [PubMed 19734654]
  49. Tributyltin induces Yca1p-dependent cell death of the yeast Saccharomyces cerevisiae., J. Toxicol. Sci., 34, 541-545, 2009.
    [J-STAGE]
  50. Heavy oil fractions induce the negative influences on mouse immune system., J. Toxicol. Sci., 34, 459-468, 2009.
    [J-STAGE]
  51. Antimicrobial activity of stereoisomers of butane type of lignans., Biosci. Biotechnol. Biochem., 73, 1806-1810, 2009.
    [PubMed 19661689]

  52. Inhibition of discoloration of yellowtail dark muscle by lignan., Biosci. Biotechnol. Biochem., 73, 1718-1721, 2009.
    [PubMed 19661688]

  53. Syntheses and antimicrobial activity of tetrasubstituted tetrahydrofuran lignan stereoisomers., Biosci. Biotechnol. Biochem., 73, 1608-1617, 2009.
    [PubMed 19584535]

  54. Abnormal response induced by pesticides on mammalian immune system., Interdisciplinary Studies on Environmental Chemistry Vol.2: Environmental Research in Asia for Establishing a Scientist's Network., 211-218, 2009.

  55. Paraquat modulates the differentiation of C2C12 cells to myotube., Interdisciplinary Studies on Environmental Chemistry Vol.2: Environmental Research in Asia for Establishing a Scientist's Network., 219-226, 2009.

  56. Severe abnormalities in the reproductive organs of mice caused by the chemical substances contained in heavy oil., J. Toxicol. Sci., 34, 239-244, 2009.
    [J-STAGE]

  57. Antimicrobial activity of stereoisomers of morinol A and B, tetrahydropyran sesquineolignan., Biosci. Biotechnol. Biochem., 73,129-133, 2009.
    [PubMed 19129639]

  58. The Effect of secoisolariciresinol on 3T3-L1 adipocytes and the relationship between molecular structure and the activity., Biosci. Biotechnol. Biochem., 73, 35-39, 2009.
    [PubMed 19129664]

  59. The structure-activity relationships of flaxseed lignan, Secoisolariciresinol., Interdisciplinary Studies on Environmental Chemistry: Biological Responses to Chemical Pollutants., 263-268, 2008.
  60. Risk assessment of heavy oil extracts on terrestrial mammals., Interdisciplinary Studies on Environmental Chemistry: Biological Responses to Chemical Pollutants., 269-274, 2008.
  61. Characterization of amino acid recycling process in yeast autophagy., Interdisciplinary Studies on Environmental Chemistry: Biological Responses to Chemical Pollutants., 241-249, 2008.
  62. Vacuolar-type H+-translocating ATPase is the target of tributyltin chloride., Interdisciplinary Studies on Environmental Chemistry: Biological Responses to Chemical Pollutants., 251-261, 2008.
  63. Antioxidant activity of butane type lignans, secoisolariciresinol, dihydroguaiaretic acid, and 7,7'-oxodihydroguaiaretic acid., Biosci. Biotechnol. Biochem., 72, 2981-2986, 2008.
    [PubMed 18997397]

  64. The mode of action of the immunostimulatory effect of collagen from jellyfish., Biosci. Biotechnol. Biochem., 72, 2806-2814, 2008.
    [PubMed 18997433]

  65. Syntheses of all stereoisomers of goniodiol from yeast-reduction products and their antimicrobiological activity., Biosci. Biotechnol. Biochem., 72, 2342-2352, 2008.
    [PubMed 18776681]

  66. Immunostimulation effect of the jellyfish collagen., Animal Cell Technology: Basic & Applied Aspects, Vol. 15, 307-313, 2008.

  67. Effects of polyamines on proliferation and IgM productivity of human-human hybridoma, HB4C5 cells., Cytotechnology, 57, 115-122, 2008.
    [PubMed 19003155]

  68. Structure-antibacterial activity relationship of 9-O,9'-O-demethyl (+)-virgatusin., Biosci. Biotechnol. Biochem., 72, 1032-1037, 2008.
    [PubMed 18391445]

  69. Stereoselective construction of tetra-substituted tetrahydrofuran compounds from benzylic hemiacetal in the presence of H2 and Pd catalyst: stereoselective synthesis of stereoisomer of (-)-virgatusin and antimicrobiological activity., Biosci. Biotechnol. Biochem., 72, 197-203, 2008.
    [PubMed 18175922]

  70. First stereoselective synthesis of meso-secoisolariciresinol and comparison of biological activity with (+) and (-)-secoisolariciresinol., Biosci. Biotechnol. Biochem., 71, 2962-2968, 2007.
    [PubMed 18071272]

  71. Use of benzyl mesylate for the synthesis of tetrahydrofuran lignan: syntheses of 7,8-trans, 7',8'-trans, 7,7'-cis, 8,8'-cis-virgatusin stereoisomers., Biosci. Biotechnol. Biochem., 71, 2248-2255, 2007.
    [PubMed 17827688]

  72. Effect of benzylic structure of lignan on antioxidant activity., Biosci. Biotechnol. Biochem., 71, 2283-2290, 2007.
    [PubMed 17827687]

  73. Antimicrobiological activity of lignin: Effect of benzylic oxygen and stereochemistry of 2,3-Dibenzyl--butyrolactone and 3,4-Dibenzyltetrahydrofuran lignans on activity., Biosci. Biotechnol. Biochem., 71, 1745-1751, 2007.
    [PubMed 17617715]

  74. Determination of the stereochemistry of the tetrahydropyran sesquineolignans morinol A and B., J. Nat. Prod., 70, 549-556, 2007.
    [PubMed 17302455]

  75. Antifungal activity of tetra-substituted tetrahydrofuran lignan, (-)-virgatusin, and its structure-activity relationship., Biosci. Biotechnol. Biochem., 71, 1028-1035, 2007.
    [PubMed 17420596]

  76. Antibacterial activity of virgatusin-related compound., Biosci. Biotechnol. Biochem., 71, 677-680, 2007.
    [PubMed 17341839]

  77. Effect of benzylic oxygen on the cytotoxic activity of phenolic lignans., Biosci. Biotechnol. Biochem., 70, 2942-2947, 2006.
    [PubMed 17151475]

  78. Immunostimulation effect of the jellyfish collagen., Biosci. Biotechnol. Biochem., 70, 2131-2137, 2006.
    [PubMed 16960386]

  79. Molecular composition of non-ionic vesicles prepared from Span 80 or Span 85 by a two-step emulsification method., J. Disper. Sci. Technol., 27, 1217-1222, 2006.

  80. The anti-tumor effect of Euchema serra agglutinin (ESA) on colon cancer cells in vitro and in vivo., Anti-Cancer Drug., 17, 943-947, 2006.
    [PubMed 16940804]

  81. Radical and superoxide scavenging activity of matairesinol and oxidized matairesinol., Biosci. Biotechnol. Biochem., 70, 1934-1940, 2006.
    [PubMed 16926506]

  82. Immunostimulation effect of the fermented milk on human hybridomas and peripheral blood lymphocytes., Animal Cell Technology: Basic & Applied Aspects, Vol. 14, 185-191, 2006.

  83. Preparation of cationic immunovesicles containing cationic peptide lipid for specific drug delivery to target cells., Cytotechnology, 47, 51-57, 2005.
    [PubMed 19003044]

  84. Immunostimulation effects of proteose-peptone component 3 fragment on human hybridomas and peripheral blood lymphocytes., Biochim. Biophys. Acta, 1725, 233-240, 2005.
    [PubMed 15978734]

  85. Gene transfection into HeLa cells by vesicles containing cationic peptide lipid., Biosci. Biotechnol. Biochem., 69, 1453-1458, 2005.
    [PubMed 16116271]

  86. First enantioselective synthesis of (-)- and (+)-virgatusin, tetra-substituted tetrahydrofuran lignan., Org. Biomol. Chem., 3, 1670-1675, 2005.
    [PubMed 15858648]

  87. Identification of a novel zebrafish SULT1 cytosolic sulfotransferase: cloning, expression, characterization, and developmental expression study., Arch. Biochem. Biophys., 347, 10-19, 2005.
    [PubMed 15820212]

  88. Anti-cancer effect to colon cancer in either vitro or vivo using lipid vesicle combined with alga lectin ESA, Congress Proceedings of 10th The Asian Pacific Confederation of Chemical Engineering Congress, 1P-01-033, 1-10, 2004.

  89. Specific targeting at ERM5-1 cell as a model human-brain tumor cell and the anti-tumor effect by using immunovesicle, Congress Proceedings of 10th The Asian Pacific Confederation of Chemical Engineering Congress, 1P-01-032, 1-9, 2004.

  90. Lactate dehydrogenase enhances immunoglobulin production by human hybridoma and human peripheral blood lymphocytes., Cytotechnology, 42, 133-143, 2003.
    [PubMed 19002935]

  91. Sulphonation of Dehydroepiandrosterone and Neurosteroids: Molecular cloning, expression, and functional characterization of a novel Zebrafish SULT2Cytosolic Sulphotransferase., Biochem. J., 375, 785-791, 2003.
    [PubMed 12885295]

  92. Sulfonation of environmental estrogens by zebrafish cytosolic sulfotransferases., Biochem. Biophys. Res. Commun., 309, 7-11, 2003.
    [PubMed 12943655]

  93. Sulfation of hydroxychlorobiphenyls: molecular cloning, expression, and functional characterization of zebrafish SULT1 sulfotransferases.,Eur. J. Biochem., 270, 2404-2411, 2003.
    [PubMed 12755695]

  94. Differential roles of human monoamine (M)-form and simple phenol (P)-form phenol sulfotransferases in drug metabolism., Arch. Biochem. Biophys., 414, 67-73, 2003.
    [PubMed 12745256]

  95. cDNA Cloning, Expression, and Functional Characterization of A Zebrafish SULT1 Cytosolic Sulfotransferase.,J. Biochem.-Tokyo, 133, 259-262, 2003.
    [PubMed 12761191] v

  96. High IgM production by human-human hybridoma HB4C5 cells cultured in microtubes., Biosci. Biotechnol. Biochem., 67, 393-395, 2003.
    [PubMed 12729006]

  97. Structure-function relationships in the stereospecific and manganese-dependent dopa/tyrosine sulfation by human M-form phenol sulfotransferase., J. Biol. Chem., 278, 1525-1532, 2003.
    [PubMed 12424257]

  98. Molecular cloning, expression, and functional characterization of a novel zebrafish cytosolic sulfotransferase., Biochem. Biophys. Res. Commun., 300, 725-730, 2003.
    [PubMed 12507510]

  99. Manganese stimulation and stereospecificity of the dopa/tyrosine-sulfating activity of human monoamine-form phenol sulfotransferase: kinetic studies of the mechanism using wild-type and mutant enzymes., J. Biol. Chem., 277, 43813-43820, 2002.
    [PubMed 12228221]

  100. Differential xenoestrogen-sulfating activities of the human cytosolic sulfotransferases: molecular cloning, expression, and purification of human SULT2B1a and SULT2B1b sulfotransferases., Biochim. Biophys. Acta,1573, 165-170, 2002.
    [PubMed 12399026]

  101. Heat denaturation enhanced immunoglobulin production stimulating activity of lysozyme from hen egg white., Biochim. Biophys. Acta, 1572, 19-24, 2002.
    [PubMed 12204328]

  102. Increase of immunoglobulin productivity of human-human hybridoma HB4C5 cells by histone., Biosci. Biotechnol. Biochem., 66, 1241-1245, 2002.
    [PubMed 12162544]

  103. The Cytotoxic effect of Eucheuma serra agglutinin (ESA) on cancer cells and its application to molecular probe for drug delivery system using lipid vesicles.,Cytotechnology., 36A93-99, 2001.
    [PubMed 19003319]

  104. Alcohol dehydrogenase-I from horse liver serves as an immunoglobulin production stimulating factor.,Enzyme Microb. Technol., 29A136-143, 2001.

  105. The mode of actions of lysozyme as an immunoglobulin production stimulating factor., Biochim. Biophys. Acta, 1475, 27-34, 2000.
    [PubMed 10806334]

  106. Effects of DNA on immunoglobulin production stimulating activity of alcohol dehydrogenase., Cytotechnology, 31, 95-102, 1999.

  107. Aldolase from rabbit muscle enhances immunoglobulin production by human hybridoma and human peripheral blood lymphocytes., Enzyme Microb. Technol., 24, 41-47, 1999.

  108. Inhibition of immunoglobulin production stimulating activity of glyceraldehyde-3-phosphate dehydrogenase by nucleotides., Biosci. Biotech. Biochem., 62, 1237-1239, 1998.
    [PubMed 9692209]

  109. A novel function of enolase from rabbit muscle; an immunoglobulin production stimulating factor., Biochim. Biophys. Acta, 1380, 163-176, 1998.
    [PubMed 9565679]

  110. Spermine enhances IgM productivity of human-human hybridoma HB4C5 cells and human peripheral blood lymphocytes., Cytotechnology, 26, 111-118, 1998.

  111. Alcohol dehydrogenase-I from horse liver stimulates immunoglobulin production by human hybridoma and human peripheral blood lymphocytes., Mol. Cell. Biochem., 173, 113-119, 1997.
    [PubMed 9278261]

  112. Lysozyme stimulates immunoglobulin production by human-human hybridoma and human peripheral blood lymphocytes., Cytotechnology, 24, 177-182, 1997.

  113. Enhancement of IgM production by human peripheral blood lymphocytes by rice fermented seasoning under serum-free cultivation., J. Ferment. Bioeng., 82, 77-79, 1996.

  114. The mode of actions of glyceraldehyde-3-phosphate dehydrogenase identified as an immunoglobulin production stimulating factor., FEBS Lett., 368, pp.92-96, 1995.
    [PubMed 7615095]


mars95@agr.ehime-u.ac.jp