Cytotechnology, 2, 63-67, 1989.


Stimulation of Monoclonal Antibody Production by Human-human Hybridoma Cells with an elevate Concentration of potassium or Sodium Phosphate in serum-free medium.

Susumu Sato, Hiroki Murakami, Takuya Sugahara, Takashi Ikegami, Koji Yamada, Hirohisa Omura, and Shuichi Hashizume


Abstract

Potassium or sodium phosphate was found to stimulate the production of human monoclonal antibody by human-human hybridoma HB4C5. The addition of 15 mM Na-phosphate (pH 7.4) into serum-free culture medium increased the antibody production up to 4-fold, when seeded at cell density of l~105 cells/ml in dishes. At the higher cell density of 5~l05 cells/ml, K-phosphate was more effective than Na-phosphate, at the same concentration. In large-scale continuous culture, the addition of l0 mM Na-phosphate into serum-free culture medium stimulated with antibody production by HB4C5 cells 6-fold.