Polymer Journal, 27, 7l9-721, 1995.


Effects of Sodium Poly(styrene sulfonate) on Antibody Production and Cell Growth of HB4C5 Hybridoma Cells.

Kazuhiko Nagiral, Midori Hayashida, Masanobu Shiga, Kazumi Sasamoto, Ken’yu Kina, Kazuhiro Osada, Takuya Sugahara, Takashi Hara, and Hiroki Murakami


Abstract

The effects of sodium Poly(styrene sulfonate) on an antibody production and cell growth of a hybridoma cell were studied. Although sodium Poly(styrene sulfonate) inhibited the antibody production and cell growth at lower concentration with increasing molecular weight in serum-free cultures, Poly(styrene sulfonate)s with medium weight-average molecular weight (Mw) such as 35000 and l8000 suppressed antibody production without inhibiting cell growth at appropriate concentrations. Poly(styrene sulfonate) (Mw=35000) decreased the extracellular concentration of a É heavy chain forming an antibody, while it had no effect on an intracellular antibody or É heavy chain concentration. This suggests that the Poly(styrene sulfonate) suppresses secreting the antibody assembled by É heavy chains and É…light chains out of the cell without inhibiting the synthesis and association of both chains in the cell.