Biotechnology and Bioengineering, 36, 759-762, 1990.

Relationship Between Oxygen Consumption Rate and Cellular Activity of Mammalian Cells Cultured in Serum-Free Media.

Koji Yamada, Shigeki Furushou, Takuya Sugahara, Sanetaka Shirahata, and Hiroki Murakami


Animal cells produce various biologically active substances such as monoclonal antibodies (MoAb), interferons, interleukins, hormones, and other biomedicals. For mass production of MoAb, serum-free, high-density culture of hybridomas can be performed successfully. To obtain cell products most efficiently, it is important to monitor cell density and activity of cells in culture vessel. However, such monitoring systems for animal cell culture have not been developed well. A promising candidate to monitor activities of cells in culture is oxygen consumption rate (OCR) of mammalian cells, though OCR differs from cell to cell and also depends on culture conditions. In this article, we examine the OCR of mouse and human lymphocytic cells in serum-free culture to find the relationship between OCR and cell activities, such as proliferative activity, cell viability, and productivity of MoAb.