Animal Cell Technology: Basic & Applied Aspects, Vol. 4, pp233-237, 1992.

Virus Containment Continuous Culture of Animal Cells.

Takuya Sugahara, Toshiaki Noda, Takashi Tsuboi, Tomoko Hirasaki, Naoki Yamamoto, Satoshi Sakaeda, and Hiroki Murakami


Human-human hybridoma HB4C5 cells were cultured in a high-density continuous culture system SHC-1 (SHIMADZU) equipped with a 500 ml culture vessel at the density of 2x107 cells/ml. Cultured medium were filtrated by the BMM hollow fiber module which can eliminate virus particles. This module was able to filtrate over 20 L of cultured medium over 30 days without decrease in the recovering rate of the cultured medium. The high density continuous culture system installed with the BMM hollow fiber can be used for virus containment in the vessel and producing virus-free products by continuous culture.