Biosci. Biotech. Biochem., 67, 393-395, 2003. [PubMed 12729006]

High IgM production by human-human hybridoma HB4C5 cells cultured in microtubes.

Takuya Sugahara, Satomi Yano, and Takeshi Sasaki


HB4C5 cells, a human-human hybridoma, were cultured in microtubes. After 24 h of cultivation, growth of the cells cultured in microtubes was 1.2- to 1.5-fold that in culture dishes or 96-well culture plates. The production of IgM was 2.6- to 3.3-fold that in the 96-well culture plates.